https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Sensitivity and antioxidant response of Chlorella sp. MM3 to used engine oil and its water accommodated fraction https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24876 Chlorella sp. MM3, to unused or used engine oil, or their water accommodated fractions (WAFs) to determine growth inhibition and response of antioxidant enzymes. Oil type and oil concentration greatly affected the microalgal growth. Used oil at 0.04% (0.4g L-1) resulted in 50% inhibition in algal growth, measured in terms of chlorophyll-a, while the corresponding concentration of unused oil was nontoxic. Similarly, used oil WAF showed significant toxicity to the algal growth at 10% level, whereas WAF from unused oil was nontoxic even at 100% concentration. Peroxidase enzyme in the microalga significantly increased with used oil at concentrations above 0.04g L-1 whereas the induction of superoxide dismutase and catalase was apparent only at 0.06g L-1. Activities of the antioxidant enzymes increased significantly when the microalga was exposed to 75 and 100% WAF obtained from used oil. The used oil toxicity on microalga could be due to the presence of toxic soluble mono- and polyaromatic compounds, heavy metals, and other compounds attained by the oil during its use in the motor engines.]]> Sat 24 Mar 2018 07:14:56 AEDT ]]> Cultivation of Chlorella on brewery wastewater and nano-particle biosynthesis by its biomass https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24773 Chlorella sp. MM3 biomass produced from the remediation of brewery wastewater. The algal growth characteristics, biomass production, nutrient removal, and nanoparticle synthesis including its characterisation were studied to prove the above approach. The growth curve of Chlorella depicted lag and exponential phase characteristics during the first 4 days in a brewery wastewater collected from a single batch of brewing process (single water sample) indicating the growth of algae in brewery wastewater. The pollutants such as total nitrogen, total phosphorus and total organic carbon in single water sample were completely utilised by Chlorella for its growth. The X-ray photoelectron spectroscopy spectra showed peaks at 706.56 eV, 727.02 eV, 289.84 eV and 535.73 eV which corresponded to the zero-valent iron, iron oxides, carbon and oxygen respectively, confirming the formation of iron nanoparticle capped with algal biomolecules. Scanning electron microscopy and particle size analysis confirmed the presence of spherical shaped iron nanoparticles of size ranging from 5 to 50 nm. To our knowledge, this is the first report on nanoparticle synthesis using the biomass generated from phycoremediation of brewery wastewater.]]> Sat 24 Mar 2018 07:14:08 AEDT ]]>